Defender Coles and midfielder Lines return to the Rovers side after completing suspensions that ruled them out of the 2-1 midweek defeat to MK Dons.
"That will strengthen us and freshen us up again," said Penney of the duo's return.
"Having new players in will give me the chance to rotate things, if I feel it's necessary."
Penney, though, is likely to be without central defender Dave McCracken and skipper Stuart Campbell as Rovers attempt to claw their way up the table and out of the relegation zone.
The pair were both forced off during the midweek home defeat with hamstring injuries, which Penney admitted was a "blow" after the game.
Source: PA
Source: PA