Colchester striker Steven Gillespie could return to the starting line-up after his appearance off the bench for the 2-1 defeat by Plymouth.
Defender Magnus Okuonghae is set to have an operation on his ankle which would rule him out for the season.
Bristol Rovers manager Dave Penney told BBC Radio Bristol: "If we make as many chances as we did on Saturday then I'm sure we'll win the match.
"Performance wise at home we've been not bad. Results could have been better and we've got to take the game to Colchester.
"We've a chance to come out of the bottom four, so there's plenty to play for."
Source: BBC Sport
Source: BBC Sport