Midfielder Lescott and full-back Hughes were struggling with knocks earlier in the week but will be available to face the Glovers.
Defender Danny Coles returns after missing the defeat at Southend last weekend ago through illness.
Boss Paul Trollope must decide whether Steve Elliott will keep his place in the centre of defence, after the former Derby man made his first League One start of the season at Roots Hall.
Elliott will compete with Coles and Byron Anthony for a starting berth, while Dominic Blizzard and Andy Williams are pushing for a place in Trollope's side.
Ben Hunt will definitely not feature due to a calf injury.
Yeovil manager Terry Skiverton is set to keep faith with his winning line-up for the clash.
As anticipated Skiverton recalled three on-loan players - who had all been on international duty - to his starting line-up for the game against Carlisle last week and was rewarded with a 3-1 home win.
Recalled Tottenham teenager Ryan Mason, who had been away with England Under 19s, scored twice, while central defender Steven Caulker, also on loan from Tottenham and on England Under-19 duty, was also recalled along with on-loan Swansea and Wales Under-21 midfielder Shaun MacDonald.
Scott Murray retained his place, following his two-goal exploits in the previous week's draw with Brighton, while defender Craig Alcock shrugged off an ankle injury to start at right back and both should be involved again.
On-loan striker Craig Davies - introduced from the sub's bench against Carlisle - has gone back to Brighton a game early.